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KMID : 0375319980200020315
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Journal of Clinical Pathology and Quality Control
1998 Volume.20 No. 2 p.315 ~ p.323
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Development and Evaluation of New Immunoturbidimetric Reagents for Apo A-I and B in the Human Serum
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Jin Q Kim
Junghan Song/Nam-Wook Jeong/Byeong-Cheol Jeon/Myung-Hoon Lee
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Abstract
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Background : The measurement of these apolipoproteins are possible by various methods such as radioimmunoassay, radial imimmunodiffusion, electroimmunoassay, immuno nephelometry and enzyme-linked immunosorbant assay. The immunoturbldimetric assay is rapid and easy to perform on automatic operation. We developed new immunoturbldi metric reagents for apo A-I and B determination.
Methods : This reagents were composed with polyclonal antisera which were raised in goat with injection of purified apo A-I and B. Each antiserum revealed no cross reactivities with other lipoproteins of human serum. To evaluate the new reagents for clinical use, we compared with other commercial reagents.
Results : In dilution test with purified HDL and LDL, we observed good linearity. In reproducibility assays, the within-run CVs were 4.3% and 3.6% and the between-run CVs were 9.0% and 5.8% for apo A-I and B respectively Average analytical recovery was 102.8% for apo A-I and 101.4% for apo B, assaying human serum samples to which we added various amounts of purified HDL and LDL. We found a good correlations with other commercial reagents. The correlation coefficients with other immunoturbidimetrIc assay reagents were r=0.922 for apo A-I and r=0.924 for apo B and with other immunonephelometric assay reagents, r=0.931 for apo A-I and r=0.895 for apo B.
Conclusions : We concluded that these reagents are accurate, precise and suitable for use in clinical laboratories.
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